Special Topics Course Announcement for Fall, 2001

Principles of Biophotonics

Physiology 675-003
Timetable

Lecture 1 Slides
Lecture 2 Slides
Lecture 3 Slides
Lecture 4 Slides
Lecture 5 Slides
Lecture 6 Slides
Lecture 7 Slides
Lecture 8 Slides [Thumbnails]  [Slide Show
Lectures 9 and 10 (see Prof. Jameson's notes)
Lecture 11 Slides [Thumbnails]  [Slide Show
Lectures 12 and 13 (see Prof. Tim Gomez's lecture notes)
Lecture 14 Slides [Thumbnails]  [Slide Show
Lecture 15 Slides [Thumbnails]  [Slide Show
Lecture 16 Slides [Slide Show
Lecture 17 Slides [Slide Show

The field of Biophotonics has emerged from research conducted at the interface of the physical and biological sciences and engineering. Correspondingly students who are interested in developing and/or applying photonic technologies to their research projects should benefit from a course that covers the basic principles of optics, optical spectroscopy and microscopy and the application of these techniques to address fundamental questions in the life and health sciences. The special topics course on Principles of Biophotonics is divided into three sections. The first part covers the basic principles and practice of photonics including optics, optical design, optical spectroscopy and optical microscopy. In the second section, students will learn how biophotonics is being used to address specific mechanisms that underlie biological function. The third section overviews novel applications of Biophotonics research in the areas of genomics, proteomics, drug screening and engineering.

Instructor: Prof. Gerard Marriott, Department of Physiology (2-8185) GM@physiology.wisc.edu

Where: 116 SMI

When: Monday and Friday: 10:00-11:30

Course Overview:

Introduction to Biophotonics

Principles of Biological spectroscopy

  • Absorption
  • Fluorescence
  • phosphorescence
  • bioluminescence

Design and performance of instrumentation used in optical spectroscopy

  • Absorption spectrophotometers
  • Fluorimeters
  • Phosphorimeters
  • Plate readers

Synthetic and natural optical probes

  • Fluorophores
  • phosphors
  • quantum dots
  • nanometer beads
  • fluorescent proteins
  • caged compounds
  • SPR

Applications of fluorescence spectroscopy in the biological sciences

  • Biomolecular interactions (binding constants, kinetics)
  • Structure and structural dynamics of biomolecules
  • Detection and analysis

The principles and practice of luminescence imaging microscopy

  • Steady-state and time-resolved fluorescence microscopy
  • Confocal microscopy: 1- and 2-photon excitation modes:
  • Steady-state and time-resolved delayed luminescence microscopy
  • Fluorescence polarization image microscopy
  • Fluorescence resonance energy transfer image microscopy
  • Single molecule image microscopy
  • Fluorescence correlation spectroscopy

Using biophotonics to study mechanisms of biomolecular reactions

  • Molecular motors: Myosin, Kinesin and F1-ATPase
  • Actin and actin filament dynamics
  • In vitro model systems
  • Kinetic investigations of complex biological processes:

Applications of fluorescence image microscopy in cell biology (Prof. Tim Gomez)

  • Review of molecular and cell biology
  • Imaging of a GFP-fusion protein in live cells
  • Other physiological indicators of cell function
  • Cell motility and cytokinesis
  • Neurobiology
  • Intravital imaging

Biophotonics in Biotechnology

  • Genomics
  • proteomics
  • high throughput screening
  • nanofabrication

Biophotonics in medicine

  • Non-invasive imaging of organs and tumors
  • Real-time monitoring of metabolites (O2, blood flow, glucose)
  • Photodynamic therapy

Each Session will include:

  1. 50 minute lecture,
  2. 20 minute review of key research papers
  3. 15 minute for review and discussion

Material: Photocopies and research papers provided by the instructor. Other material will be posted on the course web site.

Assignment:
Students will work on a written assignment following the mid-term examination. No lectures will be given during this 2-week period