Edwin R. Chapman

Return to Champman Homepage

Professor
Ph.D., University of Washington

Contact Information
Email: chapman@physiology.wisc.edu
(608) 263-1762 Phone
(608) 265-5512 Fax

Additonal Publications

• Grishanin, R.N., Kowalchyk, J.A., Klenchin, V.A., Ann K-S., Earles, C.A., Chapman, E.R., Gerona, R.R.L., and Martin, T.F.J. (2004). CAPS acts at a pre-fusion step in dense-core vesicle exocytosis as a PIP2-binding protein. Neuron 43(4): 551-62
  Abstract | Full Text | PDF
   
• Dong, M., Goodnough, M.C., Tepp, W.H., Johnson, E.A. and Chapman, E.R.. (2004). Using fluorescent sensors to detect botulinum neurotoxin activity in vitro and in living cells. Proc. Natl. Acad. Sci. USA 101(41):14701-6 Abstract | Full Text | PDF
   
• Tucker, W.T., Weber, T. and Chapman, E.R.. (2004) Reconstitution of Ca²⁺-triggered membrane fusion by synaptotagmin and SNAREs. Science 304: 435-438.
  PDF
   
• Han, X., Wang, C.T., Bai, J., Chapman, E.R. and Jackson, M.B. (2004) Transmembrane segments of syntaxin line the fusion pore of Ca²⁺-triggered exocytosis. Science 304: 289-292.
  PDF
   
• Bai, J., Wang, C.T., Jackson, M.B. and Chapman, E.R. (2004) Fusion pore dynamics are regulated by synaptotagmin*t-SNARE interactions. Neuron 41: 929-942.
  Abstract | Full Text | PDF
   
• Bai, J. and Chapman, E.R. (2004). The C2-domains of synaptotagmin: partners in exocytosis. TIBS 29(3): 143-151.
   
• Chieregatti, E., Chicka, M.C., Chapman, E.R. and Baldini, G. (2004). SNAP-23 functions in docking/fusion of granules at low Ca²⁺. Mol. Biol. Cell 15(4):1918-30.
  Abstract | Full Text | PDF
   
• Berdiev, B.K., Jovov, B., Ganeshan, H.L.R., Tucker, W., Naren, A.P., Fuller, C.M., Chapman, E.R. and Benos, D.J. (2004). The effects of syntaxin 1A on ENaC. AJP: Renal Physiology 286: F1100-F1106.
   
• Bai, J., Tucker, W.C. and Chapman, E.R. (2004). PIP2 increases the speed-of-response of synaptotagmin and steers its membrane penetration activity toward the plasma membrane. Nature Struct. Mol. Biol. 11(1): 36-44.
  Abstract | Full Text | PDF
   
• Liu, W., Montana, V., Chapman, E. R., Mohideen, U. and Parpura, V.(2003). Botulinum Toxin type B micromechanosensor. Proc. Natl. Acad. Sci. USA 100(23): 13621-13625.
  Abstract | Full Text | PDF
   
• Wang, P., Wang, C.T., Bai, J., Jackson, M.B. and Chapman, E.R. (2003). Mutations in the effector binding loops in the C2A and C2B domains of synaptotagmin I disrupt exocytosis in a non-additive manner. J. Biol. Chem. 278(47): 47030-47037.
  Abstract | Full Text | PDF
   
• Dong, M., Richards, D.A, Goodnough, M.C., Tepp, W.H., Johnson, E.A. and Chapman, E.R. (2003). Synaptotagmins I and II mediate entry of botulinum neurotoxin B into cells. J. Cell Biol. 162: 1293-1303.
  Abstract | Full Text | PDF
   
• Edwardson, J.M., Wang, C.T., Gong, B., Wyttenbach, A., Bai, J., Jackson, M.B., Chapman, E.R. and Morton, A.J. (2003). Mutant huntingtin expression inhibits exocytosis in PC12 cells by depletion of complexin II. J. Biol. Chem. 278: 30849-30853.
  Abstract | Full Text | PDF
   
• Tucker, W.C., Edwardson, J.M., Bai, J., Kim, H.J., Martin, T.F.J. and Chapman, E.R. (2003). Identification of synaptotagmin effectors via acute inhibition of secretion from cracked PC12 cells. J. Cell Biol. 162: 199-209.
  Abstract | Full Text | PDF
   
• Wang, C.-T., Lu, J.C., Bai, J., Martin, T.F.J., Chapman, E.R. and Jackson, M.B. (2003). Different domains of synaptotagmin control the choice between kiss-and-run and full-fusion. Nature 424: 943-947.
  Abstract | Full Text | PDF
   
• Wu, Y., He, Y., Bai, B., Ji, S.R., Tucker, W.C., Chapman, E.R.* and Sui, S.F.* (2003). Visualization of synaptotagmin I oligomers assembled onto lipid monolayers. Proc. Natl. Acad. Sci. USA 100; 2082-2087.
  Abstract | Full Text | PDF (*corresponding authors.)
   
• Bai, J. and Chapman, E.R. (2003). Application of fluorescent probes to determine the mechanics and dynamics of Ca²⁺-triggered synaptotagmin C2-domain-membrane interactions. Methods in Enzymology 360, 238-258.
• W.C. Tucker and Chapman, E.R. (2002). Role of synaptotagmin during Ca2+-triggered exocytosis. Biochem. J. 366, 1-13.
  Abstract | Full Text | PDF
   
• Chapman, E.R. (2002). Synaptotagmin: a Ca2+-sensor that triggers exocytosis? Nature Rev. Mol. Cell Biol. 3(7), 498-508.
  Abstract | Full Text | PDF
   
• *Bai, J., *Wang, P., and Chapman, E.R.. (2002) C2A activates a cryptic Ca2+-triggered membrane penetration activity within the C2B domain of synaptotagmin I. Proc. Natl. Acad. Sci. USA 99(3), 1665-1670. (* these authors contributed equally to this study)
  Abstract | Full Text | PDF
   
• Wang, C.-T., Grishanin, R., Earles, C.A., Chang, P.Y., Martin, T.F.J., Chapman, E.R. and Jackson, M.B. (2001) Synaptotagmin modulation of fusion pore kinetics in regulated exocytosis Science 294, 1111-1114.
  Abstract | Full Text | PDF
   
• *Earles, C., *Bai, J., Wang, P. and Chapman, E.R.. (2001) The tandem C2-domains of synaptotagmin contain redundant Ca2+ binding sites that cooperate to engage t-SNAREs and trigger exocytosis J. Cell Biology 154(6), 1117-1123. (*these authors contributed equally to this study)
  Full Text (PDF)
   
• Littleton, J.T., Barnard, R.J.O., Titus, S., Slind, J., Chapman, E.R., and Ganetzky, B. (2001) SNARE complex disassembly by NSF following synaptic vesicle fusion Proc. Natl. Acad. Sci. USA 98(21),12233-12238.
  Abstract | Full Text | PDF
   
• Lewis, J., Dong, M. and Chapman, E.R.. (2001) The transmembrane domain of syntaxin 1A is critical for cytoplasmic domain protein-protein interactions. J. Biol. Chem. 276, 15458-15465.
  Abstract | Full Text (PDF)
   
• Littleton, J.T., Bai, J., Vyas, B., Desai, R., Baltus, A.E., Garment, M.B., Carlson, S.D., Ganetzky, B. and Chapman, E.R.. (2001) synaptotagmin mutants reveal essential functions for the C2B-domain in Ca2+-triggered fusion and recycling of synaptic vesicles. J. Neurosci. 21(5) 1421-1433.
  Abstract | Full Text (PDF)
   
• Haucke, V., Wenk, M.R., Chapman, E.R., Farsad, K., and De Camilli, P.. (2000) Dual interaction of synaptotagmin with the mu2 and alpha subunits of AP-2 facilitates clathrin pit nucleation. EMBO J. 19: 6011-6019.
  Abstract | Full Text (PDF)
   
• Desai, R., Vyas, B., Earles, C., Littleton, J.T., Kowalchyck, J., Martin, T.F.J. and Chapman, E.R. (2000). The C2B-domain of synaptotagmin I is a Ca2+-sensing module essential for exocytosis. J. Cell Biology 150(5) 1125-1135.
  Full Text (PDF)
   
• Bai, J., Earles, C., Lewis, J. and Chapman, E.R. (2000). Membrane-embedded synaptotagmin interacts with cis and trans target membranes and assembles into oligomers via a novel mechanism. J. Biol. Chem. 275, 25427-25435.
  Abstract | Full Text (PDF)
   
• Davis, A.F., Bai, J., Fasshauer, D., Wolowick, M.J., Lewis, J.L. and E.R. Chapman (1999). Kinetics of synaptotagmin responses to Ca2+ and assembly with the core SNARE complex onto membranes. Neuron 24, 363-376.
  Full Text (PDF)
   
• Littleton, J.T., Sereno, T.L., Rubin, G.M., Ganetzky, B. and E.R. Chapman (1999). Synaptic function modulated by changes in the ratio of synaptagmin I and IV. Nature 400,757-760.
  Full text (PDF)
   
• Chapman, E.R., Desai, R., Davis, A.F. and Tornhel, C. (1998). Delineation of the oligomerization, AP-2- and synprint-binding region of the C2B-domain of synaptotagmin. J. Biol. Chem. 273, 32966-32972.
  Abstract | Full text (PDF)
   
• Chae, Y.K., Abildgaard, F., Chapman, E.R. and J.L. Markley. (1998). Lipid binding ridge on loops 2 and 3 of the C2A-domain of synaptotagmin I as revealed by NMR spectroscopy. J. Biol. Chem. 273, 25659-25663.
  Full Text (PDF)
   
• Littleton, J.T., Chapman, E.R., Kreber, R., Garment, M.B., Carlson, S.D. and B. Ganetzky. (1998). Paralytic mutations demonstrate that assembly and disassembly of the SNARE-complex are essential steps in neurotransmitter release. Neuron 21, 401-413.
  Full Text (PDF)
   
• Chapman, E.R. and , A.F. Davis. (1998). Direct interaction of a Ca2+-binding loop of synaptotagmin with lipid bilayers. J. Biol. Chem. 273, 13995-14001.
  Full Text (PDF)

top