University of Wisconsin - Madison
Department of Physiology, UW-Madison Medical School
Site Search
  Physiology Home  
   
News & Events
  Department News  
  Seminars & Events  
  Job Opportunities  
Education
  Graduate  
  Undergraduate  
  Affiliated Programs  
Research
  Faculty Research  
  Research Facilities  
  Research Links  
People
  Directory  
  Graduate Students  
  Administration  
  Physiology Alumni  
Faculty Resources
  Online Services  
  Campus Resources  
Contact Us
  Contact Physiology  
  Maps & Directions  
     

Baron Chanda

Baron ChandaAssistant Professor
Ph.D., 2000, National Centre for Biological Sciences, India

Contact Information
Email: chanda@wisc.edu
(608) 265-3936 (Office)
(608) 262-3520 (Lab)
(608) 265-5512 (Fax)

Research Interests
Structural mechanisms underlying voltage-dependent gating in ion channels

Visit the Chanda Lab

The objective of my research is to understand how structure and dynamics determine the function of the voltage-dependent ion channels. In response to a change in membrane potential, voltage-dependent ion channels undergo a series of conformational changes culminating in the opening or closing of the channel. We use a variety of biophysical methods to track the structural dynamics of these processes. Electrophysiological methods like ionic and “gating” current measurements provide information about the global structural changes. Site-specific fluorescence recordings and cysteine accessibility methods, on the other hand, provide information about the local structural changes in the protein. These methods complement one another and together they provide a detailed physical model of the workings of an ion channel.

Figure 1We are currently focusing on understanding how the voltage-dependent gating behavior of sodium channels is modified by gating-modifier toxins and local anesthetics. Sodium channel malfunction has been associated with disease conditions like cardiac arrhythmias and epilepsies. Local anesthetics alleviate these disease states by reducing electrical excitability of the sodium channels. We are using voltage-clamp fluorimetry to map the structural changes in the sodium channels induced by local anesthetics. These studies are expected to provide fundamental insights into the physical mechanisms of gating and modulation of the Na+ channel.

Another area of interest in the lab is to understand how temperature modulates voltage-dependent gating of TRP ion channels. Some members of the TRP family respond acutely either to a heat or to a cold stimulus. Recently, it has been shown that these channels are also voltage-dependent and that temperature affects their voltage-dependent gating. We are interested in understanding the biophysical principles that govern temperature dependent gating of these channels using both time-resolved spectroscopic as well as electrophysiological approaches.

Figure 2

Selected Publications

  • Campos, F., Chanda, B.d, Baeiro, P., and Bezanilla, F.d (2007) b-scorpion toxin modifies gating transitions in all four voltage-sensors of the sodium channel. J. Gen. Physiol. 444(7115), 10.1085/jgp.200609719.
     
  • Ben-Chaim, Y., Chanda, B., Bezanilla, F., Dascal, N., Parnas, I. and Parnas, H. (2006) Movement of “gating charge” is coupled to ligand binding in a G-protein coupled receptor. Nature 444(7115), 106-109.
     
  • Bannister, J.P., Chanda, B., Bezanilla, F., and Papazian, D.M. (2005) Optical detection of rate-determining, ion-modulated conformational changes of the ether-ŕ-go-go K+ channel voltage sensor. Proc. Natl. Acad. Sci. (USA) 102(51),18718-19723.
     
  • Chanda, B.*, Blunck, R.*, Faria, L.C., Schweizer, F.E., Mody, I. and Bezanilla, F. (2005) A hybrid approach to measuring electrical activity in genetically specified neurons. Nature Neuroscience 8 (11), 1619-1626.
     
  • Chanda, B., Asamoah, O.K., Blunck, R., Roux, B. and Bezanilla, F. (2005) Gating charge displacement in voltage-gated ion channels involves limited transmembrane movement. Nature 436(7052), 852-836.
     
  • Chanda, B.*, Asamoah, O.K.*, and Bezanilla, F. (2004) Coupling interactions between voltage sensors of the sodium channel as revealed by site-specific measurements. J. Gen. Physiol. 123, 217-230.
     
  • Chanda, B. and Bezanilla, F. (2002) Tracking voltage-dependent conformational changes in skeletal muscle sodium channel during activation. J. Gen. Physiol. 120, 629-645.

* = Equal contribution
d = Corresponding authors

   
 
UW Home | Medical School | Physiology Home
 
 

Department of Physiology
University of Wisconsin
1300 University Avenue, Room 125 SMI
Madison, Wisconsin 53706-1510
Phone: (608) 262-2938
Fax: (608) 265-5512

Administrative Contact: office@physiology.wisc.edu
Web Contact: webmaster@physiology.wisc.edu

Last Updated: Feb. 22, 2011